0900 - Effect of Insulin-like Growth Factor-i on Total Collagen Content Synthesized by Human Chondrocytes Cultured in Alginate Beads
نویسندگان
چکیده
In an attempt to repair chondral lesions in a joint, different methods are under investigation that use human chondrocytes for transplantation. In our laboratory we have used the alginate system to study the effects of various growth factors and cytokines on human chondrocyte metabolism [1]. Evidence is accumulating that Insulin-like growth factor-I (IGF-I) may play a key role in maintaining the synthetic activity of adult articular cartilage [2]. Our recent studies have been focused on the potential role of exogenous IGF-I in short-term culture using alginate beads as a three-dimensional support to determine the effects of IGF-I on cell proliferation and total collagen content.
منابع مشابه
Response of human chondrocytes prepared for autologous implantation to growth factors.
This study investigated metabolism of autologous chondrocytes after initial expansion immediately before implantation. Chondrocytes cultured in either monolayers or alginate beads were treated with insulin-like growth factor-1 (IGF-1), osteogenic protein-1 (OP-1), or a combination. Proteoglycan synthesis and DNA content were tested in both cultures. Alginate beads also were analyzed with live/d...
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